Data are expressed as mean ± SD of three independent experiments. The data show percentage of cell number in G0/G1, S, and G2/M cell cycle phases. B Cell cycle analysis in non-targeting control (NTC) hESC-derived cells at the indicated stage of thyroid differentiation lineage. Treatment with FGF10, KGF, BMP4 and EGF is reported as FKBE. To promote thyroid lineage specification, hESCs were exposed to the indicated stimuli. Increasing radioiodine uptake, KISS1R and TIMP1 targeting may represent a therapeutic adjuvant option for undifferentiated TCs.Ī Model of directed differentiation of human embryonic stem cell (hESC) into thyroid lineage. Tissue Inhibitor of Metalloproteinase 1 (TIMP1)/Matrix metallopeptidase 9 (MMP9)/Cluster of differentiation 44 (CD44) ternary complex, in cooperation with Kisspeptin receptor (KISS1R), is involved in TC initiation and progression. The same mutations result in teratocarcinomas when delivered in early differentiating hESCs. Of note, TCs arise by engineering TPCs, whereas mature thyrocytes have a very limited tumorigenic capacity. Specifically, TPCs harboring BRAF V600E or NRAS Q61R mutations generate papillary or follicular TC, respectively, whereas addition of TP53 R248Q generate undifferentiated TCs. Here, we create follicular cell-derived TCs of all the different histotypes based on specific genomic alterations delivered by CRISPR-Cas9 in hESC-derived TPCs. Human embryonic stem cells (hESCs) with appropriate in vitro stimulation undergo sequential differentiation into thyroid progenitor cells (TPCs-day 22), which maturate into thyrocytes (day 30). The cell subpopulation in the lineage hierarchy that serves as cell of origin for the different TC histotypes is unknown. carcinoma (TC) is the most common malignancy of endocrine organs. 9 Department of Surgical Oncological and Stomatological Sciences, University of Palermo, Palermo, Italy.1 Department of Surgical Oncological and Stomatological Sciences, University of Palermo, Palermo, Italy. "Paolo Giaccone", University of Palermo, Palermo, Italy. 7 Department of Experimental Oncology, Mediterranean Institute of Oncology, Viagrande, Catania, Italy.6 Endocrinology Unit, Department of Clinical and Experimental Medicine, University of Catania, Garibaldi-Nesima Hospital, Catania, Italy.5 Queen Mary University, Experimental Medicine & Rheumatology, London, United Kingdom. 4 Villa Sofia-Cervello Hospital, Palermo, Italy.Policlinico-Vittorio Emanuele, Center of Experimental Oncology and Hematology, University of Catania, Catania, Italy. 3 Department of Clinical and Experimental Medicine, A.O.U.2 Department of Health Promotion Sciences, Internal Medicine and Medical Specialties, University of Palermo, Palermo, Italy.1 Department of Surgical Oncological and Stomatological Sciences, University of Palermo, Palermo, Italy.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |